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Biotechnological Laboratory    

 

We emphasise to use several up-to-date biotechnological techniques to support both routine and specific jobs as stated on the table. These are performed as a tool to strengthen and increase the accuracy, specificity and reliability of the laboratory results.

 

 Biotechnological techniques Purpose/ Objective 
 1. DNA fingerprint
  •  Characterise and determine an animal individual’s identity
  • Perform in cattle, buffaloes, goats and chickens
2. Parentage analysis
  •  Examine the paternity, maternity or both of a child (paternity and maternity tests) by using DNA fingerprint technique
  • Perform in cattle and buffaloes
3. Detection of genetic diseases related reproductive performance 
  •  Identify genetic disease’ s carrier which affects herd reproductive problems and infertility including bovine leukocyte adhesion deficiency (BLAD), complex vertebral malformation (CVM), Factor 9 deficiency, DAMPs
  • Benefit for Tropical Holstein dairy cattle project and the selection process of bull dams 
4. Detection of disease resistant genes
  •  Determine animal carried resistant gene
  • Benefit for animal selection process and disease control 
5. Detection of meat quality controlling genes
  •  Analyse genes controlling meat quality in terms of tenderness, juiciness and marbling 
6. Species characterisation
  •  Classify individual animal species
  • Perform in ruminants (cattle, buffaloes, goats and sheeps)/ chickens/ pigs
7. Breed characterisation
  •  Classify and characterise breed of each species
  • Benefit for breed registration and the study of animal diversity
8. Mitochondrial DNA genotyping
  •  Identify mitochondrial DNA sequence variations in each species
  • Help determining the maternal lineage of an individual
9. Recombinant DNA genotyping
  •  To produce positive control samples which are suitable for identifying genetic diseases related poor reproductive performance in animal
10. Chromosomal analysis and karyotype
  •  Identify and evaluate the size, shape and number of chromosome
  • Determine various chromosomal abnormalities including missing chromosome, abnormal positions of chromosomal pieces which can affect the reproductive performance 

11. Early pregnancy diagnosis in milk and blood samples

11.1 Hormone level testing

 

11.2 Bovine pregnancy protein testing 

 

  • Evaluate blood progesterone level during the early pregnancy period in order to predict the pregnancy status or determine other reproductive system abnormalities, such as cystic ovary 

 

  • Detect the level of bovine pregnancy- associated protein as a marker for pregnancy by using ELISA technique
  • Increase the accuracy and specificity compared to the conventional method   
12. Embryo transfer technology
  •  Assist the reproductive performance by placing embryos into the uterus of a female recipient by using fresh or frozen embryos (may be used in connection with in vitro fertilization; IVF)
  • Introduce selected genetic material into livestock populations, allow top quality female animal to have a greater influence on the herd genetic advancement, reduce the risk for transmission of reproductive infectious diseases 

 

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This diagram represents the application of biotechnological laboratories on animal selection, animal diversity and molecular forensic which involve with the upcoming establishment of gene and germplasm bank and bioinformatics system.    

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